关键词: 层黏连蛋白, 姜黄素, HepG-2细胞, 免疫印迹法, 流式细胞术

Abstract: Objective To study the influence of curcumin on cell growth and apoptosis in HepG-2 cell, in the presence of exogenous laminin(LN) to its receptor. Methods HepG-2 cells were cultured in DMEM containing 10% fetal bovine serum at 37℃ in a humidified atmosphere with 5% COSUB>2./SUB>The experiment was performed in four groups:the control group, LN group(20μg/L LN), curcumin group(40μmol/L curcumin),and combination group ( 20μg/L LN+40μmol/L curcumin ). Acid phosphatase assay(APA),flow cytometry（FCM） and Western blotting were used to detect the cell viability, apoptosis ratio, intracellular calcium concentration ,mitochondrial transmembrane potential,expression of proliferation-related proteinα-PKC and apoptosis-related protein poly ADP ribose polymerase(PARP), Caspase-3, p53 and Bcl-2 Results Compared with the control group ,LN group increased the viability rate. Curcumin(40μmol/L)and combination( 20μg/L LN+40μmol/L curcumin ) inhibited HepG-2 cells proliferation obviously, somewhat in a time dependent manner. After treatment with 40μmol/L curcumin and 20μg/L LN+40μmol/L curcumin for 48hours,the number of HepG-2 cells reduced, volums shrank, shape rounded and suspended mostly. The rates of late apoptosis and necrosis cells(%) were 97.04±1.50,98.02±1.35.Intracellular calcium concentration rised. Mean fluorescence intensity (MFI) of mitochondrial transmembrane potentials in HepG2 cells dropped. The expression of proliferation-related proteinα-PKC decreased. Curcumin could induce cleavage of PARP. The expression of apoptosis-related protein p53 increased, Caspase-3 decreased but Bcl-2 ha

Key words: Laminin, Curcumin, HepG-2 cell, Western blotting, Flow cytometry